Figure 7

Progress in the development of Lipoplex and Polyplex modified with Anionic Polymer for efficient Gene Delivery

Yoshiyuki Hattori*

Published: 24 July, 2017 | Volume 1 - Issue 1 | Pages: 003-018

jgmgt-aid1002-g007

Figure 7:

Effect of chondroitin sulfate (CS) or poly-α-glutamic acid (αPGA) on the biodistribution of small interfering RNAs (siRNAs) in mice after intravenous injection of DOTAP/Chol lipoplexes [86-88]. In sequential injection, at 1 min after intravenous injection of 1 mg CS or PGA, DOTAP/Chol lipoplexes with Cy5.5-labeled siRNA (Cy5.5-siRNA) were administered intravenously to mice [86-88]. Ex vivo images of dissected tissues (A) and tissue sections (lung and liver) (B) were obtained at 1 h after injection of siRNA lipoplexes. In A, fluorescence intensity is illustrated using a color-coded scale (red is maximum, purple is minimum). In B, green signals indicate the localization of Cy5.5-siRNA. Scale bar=100 µm.

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